Updated on 19 October, 2021
The Coliforms Test is one of the most frequently used diagnostic laboratory tests in clinical and research laboratories worldwide. The test has evolved from its original role as a method to determine the bacterial count in human blood, urine or stool samples. Originally, the test was designed to be able to detect infections in the GI tract (enteritis, cholecystitis and irritable bowel syndrome) in addition to blood cell samples taken for various diseases. However, recent studies have revealed that the Coliforms Test can also detect bacterial diversity in non GI tissues, such as eye wash samples, mouth wash samples and skin scrapings. Furthermore, it can also detect potentially harmful organisms in non-Grain fiber foods and beverages.
The use of the Coliforms Test is based on the concept of mass culture of micro-organisms to identify those involved in pathogenicity and health threatening conditions. For example, it is used to evaluate the presence of e-coli in drinking water. This indicator can be detected in both fresh and salt water supplies. To determine the exact amount of e-coli in drinking water samples, a sample is drawn from a specific area in a body area with known e-coli prevalence.
In previous studies, the average percentage of e-coli present in total coliforms was approximately 10%. In addition, the majority of these bacteria are known to be of a warm-blooded animals origin, which means animals with large amounts of lymphoid tissue such as cattle, deer, fish and sheep typically carry a higher concentration of e-coli. Based on the results of the Total Coliform Test, doctors determine if an individual may be at risk of developing a health condition associated with e-coli contamination of the GI tract.
According to scientists, there are several reasons why e-coli may be present in the human body. The greatest reason is an increase in the number of anaerobic pathogens in the gut, which live directly in the intestinal tract. When these anaerobic pathogens grow rapidly, they release toxins into the intestine, which in turn activates the immune system to combat these pathogens. However, if the immune system is already weakened due to other causes, or the E-coli are growing very quickly in the gut, the toxins produced by the bacteria can cross the intestinal lining and reach the bloodstream.
As soon as this happens, there are several indicators that will appear in the blood. First, the total coliforms test results will show high levels of particulates in the feces. These particulates are the remains of the anaerobic bacteria that have broken down. After they break down, the bacteria produce gases that are often detected by this indicator. Also, when the bacteria metabolize proteins, the indicator will become more sensitive to changes in the amino acid composition of proteins. If there is a drastic change in these values, it means that the E-coli have reached the intestines and can be detected through blood tests.
In addition, the total coliforms test results can also indicate the presence of Giardia, another common cause of diarrhea. The indicator in the blood will become more sensitive if the E-coli are present in the gastrointestinal tract. If the E-coli are detected in the blood, then the doctor will perform a blood culture to confirm the diagnosis of E-coli. However, this indicator does not indicate the presence of other pathogenic organisms in the digestive tract. This would require a sample of the stool or the blood.
To eliminate the possibility that the blood sample results are being affected by E-coli, it is important to have samples from all body fluids. This includes the urine, the feces, the blood, the cerebrospinal fluid, and the hot tubs. These samples will be subjected to different techniques and they will provide more detailed results. This method can distinguish between various indicators organisms, and this will also help detect the presence of E-coli in hot tubs and other body fluids.
Ultrasound, Uv light, and chemiluminescent immunoassays can also be used in detecting the presence of coliforms. The main advantage of using these techniques is that they can determine the exact amount of the bacteria to be identified. Coliforms are detected in hot tubs, drinking water, taps, and other areas where there are masses of water. Coliforms appear as dark spots, white stripes, or as flat or slightly elevated formations in the water. To detect the presence of E-coli, special tools are required in the form of hot tubs and UV light, and these tools are available in a variety of sizes.